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M9490589.TXT
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1994-09-24
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Document 0589
DOCN M9490589
TI [Detection of HTLV-1 provirus DNA in T-cell neoplasms]
DT 9411
AU Takenaka T; Kuribayashi K; Tsukiyama M; Nakamine H; Maeda J; Hiroi K;
Kuno T; Nakamura Y; Department of Laboratory Medicine, Wakayama Medical
School.
SO Rinsho Byori. 1994 Jul;42(7):725-30. Unique Identifier : AIDSLINE
MED/94343730
AB Using polymerase chain reaction (PCR) amplification and Southern
hybridization, gag, pol, env and pX region genes of HTLV-1 provirus were
detected in T-cell malignancy such as adult T-cell leukemia/lymphoma
(ATLL), mycosis fungoides in leukemic phase (MF) and, CD8-leukemia. The
gag, pol, and/or env regions, were not amplified in some cases of ATLL,
which was considered to be induced by mutation, but not deletion.
However, the pX-1 and pX-2 regions could be amplified in all cases
examined. As it is suggested that the pX gene plays an important role in
leukemogenesis, the mutation may not occur in this region gene.
Interestingly, the pX-2 was amplified in the cases with MF and
CD8-leukemia as well. The amplified products were hybridized to the
HTLV-1 pX sequence, even though the products contained DNA fragments
with a size larger than expected as well as those of an expected size.
These results indicated the possibility that the virus having sequence
identical with HTLV-1 pX is integrated in the tumor cells of MF and CD8
leukemia.
DE Base Sequence DNA, Viral/*ANALYSIS English Abstract Human
HTLV-I/GENETICS/*ISOLATION & PURIF Leukemia-Lymphoma, T-Cell, Acute,
HTLV-I-Associated/*MICROBIOLOGY Molecular Sequence Data Mycosis
Fungoides/MICROBIOLOGY Polymerase Chain Reaction
Proviruses/GENETICS/*ISOLATION & PURIF Skin Neoplasms/MICROBIOLOGY
JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).